thuringiensis strains were often observed. anthracis, cross-reactions with closely related B. ![]() Although many of the chromosomal targets evaluated are claimed to be specific to B. Published primer and probe sequences were compared for specificity against 134 available Bacillus spp. Following a review of the literature, an in silico analysis of all signature sequences reported for identification of B. For the detection of chromosomal DNA, different genes have been used, such as BA813, rpoB, gyrA, plcR, S-layer, and prophage-lambda. While most of the developed assays rely on unique markers present on virulence plasmids pXO1 and pXO2, relatively few assays incorporate chromosomal DNA markers due to the close relatedness of B. There are many PCR-based assays in use for the detection of B. ![]() Bacillus anthracis, the causative agent of anthrax, is a zoonotic pathogen that is relatively common throughout the world and may cause life threatening diseases in animals and humans.
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